| Gene | Ccl26 |
| Chromosome | 5 |
| Deletion Start | 135,563,566 |
| Deletion End | 135,560,445 |
| Deletion Size | 3,122 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 135,563,286 | 135,563,307 | GACGGTGTGATGTGAGTGTTTC |
| TUP | 135,563,263 | 135,563,285 | TCAGTCCAGCTTCATCGGCCACA |
| TUR | 135,563,203 | 135,563,221 | CCCATCTTGAGGCCCAGTC |
| TDF | 135,560,769 | 135,560,792 | ACAGATTACTGCCTGACAACTAAC |
| TDP | 135,560,749 | 135,560,767 | AAACCTTCTGAGACATGAT |
| TDR | 135,560,720 | 135,560,741 | ACACACCCTCAAGCCCACATTC |
PCR Primers
| Primer | Sequence |
| SU | TATAGGACATGAGCCAGGTG |
| SD | GCTACTTTACACCATGTGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 324 bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | 330 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11452A-E5 | VGB6 | C57BL/6NTac | |
| 11452A-F1 | VGB6 | C57BL/6NTac | |
| 11452A-A8 | VGB6 | C57BL/6NTac | |
| 11452A-G8 | VGB6 | C57BL/6NTac | |
| 11452A-G12 | VGB6 | C57BL/6NTac | |
