| Gene | Rnf182 |
| Chromosome | 13 |
| Deletion Start | 43,667,957 |
| Deletion End | 43,668,725 |
| Deletion Size | 769 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,668,265 | 43,668,286 | GAAGAAATGCCTGCCTGAGAAC |
| TUP | 43,668,294 | 43,668,317 | AGCTGTTGCTCACTCCCAAGAGGC |
| TUR | 43,668,321 | 43,668,340 | GGAAGGGCTGACAAGGGAAG |
| TDF | 43,668,457 | 43,668,475 | CACCACCGTGTCCCATAAC |
| TDP | 43,668,476 | 43,668,498 | TGGACGGTGTGGAACTGCACCTC |
| TDR | 43,668,548 | 43,668,568 | GGGCAAGGAGCTGAAGTACAG |
PCR Primers
| Primer | Sequence |
| SU | CAAACCTGTCAGAGACGGC |
| SD | TCTGGATAGGAAAACGGACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 302 bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 112 bp | + | + | - |
| NeoFwd | SD | 224 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11455A-A1 | VGB6 | C57BL/6NTac | |
| 11455A-F3 | VGB6 | C57BL/6NTac | |
