| Gene | Piwil4 |
| Chromosome | 9 |
| Deletion Start | 14,740,730 |
| Deletion End | 14,702,340 |
| Deletion Size | 38,391 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 14,740,482 | 14,740,501 | GCCACAGTGCTAGGGAAGTG |
| TUP | 14,740,451 | 14,740,478 | CGGTCCTCAAGAGACCTCATGGTGAGTA |
| TUR | 14,740,372 | 14,740,394 | TGGGAAAGCCTCAAAGGAGTTAG |
| TDF | 14,702,958 | 14,702,978 | TGTCGTGCCCTTTCTAAACTC |
| TDP | 14,702,933 | 14,702,957 | TGTCCACTCTGCACGCCTAACTTCA |
| TDR | 14,702,913 | 14,702,932 | CGTGTACCCACTCCCTTCTG |
PCR Primers
| Primer | Sequence |
| SU | AGCACACCTTATGACCGTCC |
| SD | CCACATGAGAATTTACCTCAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 130 bp | + | + | - |
| TDF | TDR | 66 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11456A-C5 | VGB6 | C57BL/6NTac | |
| 11456A-E1 | VGB6 | C57BL/6NTac | |
