| Gene | Zfp775 |
| Chromosome | 6 |
| Deletion Start | 48,613,215 |
| Deletion End | 48,620,816 |
| Deletion Size | 7,602 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 48,613,982 | 48,614,002 | CAGTGGGCAGATGGAGAAGAG |
| TUP | 48,614,010 | 48,614,035 | CAGGGTCGTCTTCCTGGTTGGATGTC |
| TUR | 48,614,041 | 48,614,063 | AATGCCTGGCCTGATTAAACTTG |
| TDF | 48,620,000 | 48,620,019 | CCGCTTAGGTCCCAGTGAAC |
| TDP | 48,620,022 | 48,620,047 | CGCCAGTTCATCTGCAATGATTGCGG |
| TDR | 48,620,082 | 48,620,099 | CGTGTGGATGCGCTGATG |
PCR Primers
| Primer | Sequence |
| SU | GCTATGAGATGGGACATGG |
| SD | CCACAGCTTTGATCTCACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 262 bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | 258 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11457A-F9 | VGB6 | C57BL/6NTac | |
| 11457A-D9 | VGB6 | C57BL/6NTac | |
| 11457A-B11 | VGB6 | C57BL/6NTac | |
