| Gene | Neurl3 |
| Chromosome | 1 |
| Deletion Start | 36,273,362 |
| Deletion End | 36,266,316 |
| Deletion Size | 7,047 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,273,260 | 36,273,279 | CAGGTGGGAGGTCTGGAAAG |
| TUP | 36,273,225 | 36,273,253 | TCAGAGACTTCATTGGCTCCTAGAGTGGG |
| TUR | 36,273,177 | 36,273,195 | GGGAAGGCAGGAAGCTCTC |
| TDF | 36,266,392 | 36,266,411 | GATCGAGGAGGTGGCTGTAG |
| TDP | 36,266,365 | 36,266,391 | TGTCTTCACTGAAGGCTGAGGAAGGCT |
| TDR | 36,266,333 | 36,266,353 | GTCCCATCTGCCCAAGAAGTC |
PCR Primers
| Primer | Sequence |
| SU | CTGTGAGCCACGAGAGGTC |
| SD | CATGTCAGAACCTCCTGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 310 bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | 250 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11458A-F4 | VGB6 | C57BL/6NTac | |
| 11458B-D3 | VGB6 | C57BL/6NTac | |
