| Gene | Sprr3 |
| Chromosome | 3 |
| Deletion Start | 92,457,532 |
| Deletion End | 92,456,780 |
| Deletion Size | 753 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 92,457,473 | 92,457,493 | CAGCCTGAACAGCATCAAGTG |
| TUP | 92,457,447 | 92,457,471 | AGCAACCTTGTCAGCCTCCACCACA |
| TUR | 92,457,417 | 92,457,436 | TCAGAGGTGGCTATGGGAAC |
| TDF | 92,456,974 | 92,456,996 | ACCTGGCTACATGAATGCCTCAG |
| TDP | 92,456,949 | 92,456,971 | CCCACCCATGCCAAGGTTCCTGA |
| TDR | 92,456,919 | 92,456,939 | CCCTGGTCAGGTATCTTGGTG |
PCR Primers
| Primer | Sequence |
| SU | TCTTGTTGAGAACTTTCACCATC |
| SD | TCTTGACTCACCAAGGAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11464A-C4 | VGB6 | C57BL/6NTac | |
| 11464A-D1 | VGB6 | C57BL/6NTac | |
