| Gene | Dok7 |
| Chromosome | 5 |
| Deletion Start | 35,064,348 |
| Deletion End | 35,087,202 |
| Deletion Size | 22,874 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 35,064,675 | 35,064,694 | TGGGTGTCTCAGGGTCAAAG |
| TUP | 35,064,697 | 35,064,716 | CCAGGCAGGCCCTTGCAGGT |
| TUR | 35,064,735 | 35,064,755 | GCCACGGCACTGAGTTCATTC |
| TDF | 35,087,007 | 35,087,025 | TCCCGCTATGCCCAGATTG |
| TDP | 35,087,027 | 35,087,053 | CATTGCAGCTACCGAGACAGCACATCG |
| TDR | 35,087,099 | 35,087,116 | TTCTTCCGCTGCTCCAAC |
PCR Primers
| Primer | Sequence |
| SU | GTGGCAGATGAGAAATGCAC |
| SD | GATGCTGAGGCTACCGATTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 522 bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | 369 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11471A-A3 | VGB6 | C57BL/6NTac | |
| 11471A-E12 | VGB6 | C57BL/6NTac | |
| 11471A-D5 | VGB6 | C57BL/6NTac | |
