| Gene | Ubqln3 |
| Chromosome | 7 |
| Deletion Start | 104,142,878 |
| Deletion End | 104,140,879 |
| Deletion Size | 2,000 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 104,142,631 | 104,142,650 | CAGTGTGGAGTGCGAGATGG |
| TUP | 104,142,602 | 104,142,628 | TCACAGTCCACCTGGTCATCAAGATGC |
| TUR | 104,142,551 | 104,142,574 | CTGGAATAGAGACTGGTGGTGATG |
| TDF | 104,140,979 | 104,141,001 | GGCTATGGGCTTTCTGAATCTTG |
| TDP | 104,140,954 | 104,140,978 | AAGCCAATCTCCAGGCCCTCATTGC |
| TDR | 104,140,922 | 104,140,943 | TCTCCACAGCAGCATCTACATC |
PCR Primers
| Primer | Sequence |
| SU | AGCAAGATGAGGGCAGTTTG |
| SD | AAGCCTTTGGCTCCTTTAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11472B-H12 | VGB6 | C57BL/6NTac | |
| 11472B-C9 | VGB6 | C57BL/6NTac | |
| 11472B-G1 | VGB6 | C57BL/6NTac | |
| 11472B-B1 | VGB6 | C57BL/6NTac | |
| 11472C-E4 | VGB6 | C57BL/6NTac | |
| 11472C-G12 | VGB6 | C57BL/6NTac | |
