| Gene | Sh2d4b |
| Chromosome | 14 |
| Deletion Start | 40,892,962 |
| Deletion End | 40,872,535 |
| Deletion Size | 20,428 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 40,892,642 | 40,892,662 | GGATTTGGAAGCCTTGTGCTG |
| TUP | 40,892,611 | 40,892,638 | CCTTTAGTCCCACTAGCTTGGGTTTCCA |
| TUR | 40,892,576 | 40,892,595 | GCATAGTCCCTGCCACATTC |
| TDF | 40,873,472 | 40,873,494 | GCTCACAATGGCAAGTCCTTTAC |
| TDP | 40,873,438 | 40,873,467 | TTAACTGTTTCCATACATAGCCCAAGCCAC |
| TDR | 40,873,408 | 40,873,429 | GGAAGACCATCCTGGAGGAAAC |
PCR Primers
| Primer | Sequence |
| SU | TGGGTCTGAGCAGATGTTTG |
| SD | AAAGATGGGCCTAAGGTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 492 bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | 268 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11478A-B8 | VGB6 | C57BL/6NTac | |
| 11478A-C1 | VGB6 | C57BL/6NTac | |
| 11478A-H4 | VGB6 | C57BL/6NTac | |
| 11478A-D7 | VGB6 | C57BL/6NTac | |
| 11478A-F10 | VGB6 | C57BL/6NTac | |
