| Gene | Prss2 |
| Chromosome | 6 |
| Deletion Start | 41,521,788 |
| Deletion End | 41,525,024 |
| Deletion Size | 3,237 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 41,522,560 | 41,522,583 | TCAGGTAATTCTTGCTGTCCCATC |
| TUP | 41,522,585 | 41,522,611 | CGGATGCTCAGAAGAGATCTGGGAACA |
| TUR | 41,522,646 | 41,522,667 | GGCTTCCCAGTATCAGGTTCTG |
| TDF | 41,524,359 | 41,524,378 | GGTGGCTACCCATGACATTC |
| TDP | 41,524,385 | 41,524,411 | AAACTCTTGTTCCTCCAAGTGCGCTGA |
| TDR | 41,524,454 | 41,524,473 | GGAGCAGGTCTGGGTTGTTC |
PCR Primers
| Primer | Sequence |
| SU | TCCTCCTTATGTGACCTGAG |
| SD | GTGTTTCCTATGCTGCTGTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 349 bp | - | + | + |
| TUF | TUR | 108 bp | + | + | - |
| TDF | TDR | 115 bp | + | + | - |
| NeoFwd | SD | 286 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11483A-B6 | VGB6 | C57BL/6NTac | |
| 11483A-C8 | VGB6 | C57BL/6NTac | |
| 11483A-F5 | VGB6 | C57BL/6NTac | |
| 11483A-F11 | VGB6 | C57BL/6NTac | |
| 11483A-F7 | VGB6 | C57BL/6NTac | |
