| Gene | Lrrc30 |
| Chromosome | 17 |
| Deletion Start | 67,632,580 |
| Deletion End | 67,631,652 |
| Deletion Size | 929 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 67,632,452 | 67,632,471 | ACCCACGGTCCTTGCTGAAG |
| TUP | 67,632,424 | 67,632,446 | CATGCGGCACGTCAGCTTCAGTC |
| TUR | 67,632,338 | 67,632,359 | GAGCTGGTTGTGGGACAGATTG |
| TDF | 67,631,797 | 67,631,815 | GGGAGGATTGCGTGGAACC |
| TDP | 67,631,771 | 67,631,796 | CCATGGACAAAGGGCTGCACATTTCC |
| TDR | 67,631,744 | 67,631,763 | CGGCAAGGGCTTGGATAAAG |
PCR Primers
| Primer | Sequence |
| SU | GCTCTCCTGCAACCATGTAG |
| SD | AGTCACACTTTGAGTGAGATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 297 bp | - | + | + |
| TUF | TUR | 134 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | 318 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11484A-D1 | VGB6 | C57BL/6NTac | |
| 11484A-H11 | VGB6 | C57BL/6NTac | |
| 11484B-B1 | VGB6 | C57BL/6NTac | |
| 11484B-H3 | VGB6 | C57BL/6NTac | |
| 11484B-G9 | VGB6 | C57BL/6NTac | |
| 11484B-F1 | VGB6 | C57BL/6NTac | |
| 11484B-C8 | VGB6 | C57BL/6NTac | |
