11485   Foxi2     Status: ES cell colonies screened / QC positives

GeneFoxi2
Chromosome7
Deletion Start135,410,370
Deletion End135,412,053
Deletion Size1,684
Genome Build38
MGI IDMGI:3028075
NCBI Gene ID270004
BAC ID303G3
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 7

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF135,410,872135,410,891ACTCCATCCGCCACAACCTG
TUP135,410,893135,410,920CGCTCAATGATTGCTTCAAGAAGGTGCC
TUR135,410,928135,410,951CTGCTGTTTACCTGGATCGTTCTC
TDF135,411,428135,411,448TAGCCCATGCTTGGATCTTTC
TDP135,411,449135,411,469TGGCGCACCCAAATTCTTGCA
TDR135,411,493135,411,513CCTTGAGGGTTCACTGTGTTG

PCR Primers

PrimerSequence
SUACCTAACAGAGAGACCTAAG
SDAGAGCTGGATCTATAGGCAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev341 bp-++
TUFTUR80 bp++-
TDFTDR86 bp++-
NeoFwdSD269 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
11485A-F3VGB6C57BL/6NTac
11485A-F4VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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