| Gene | Rnf133 |
| Chromosome | 6 |
| Deletion Start | 23,650,054 |
| Deletion End | 23,648,895 |
| Deletion Size | 1,160 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 23,649,681 | 23,649,701 | ATCTGAGCACGGAGCCAGAGG |
| TUP | 23,649,642 | 23,649,667 | AATTTCCCAGGTACTGGCAACCAGGT |
| TUR | 23,649,610 | 23,649,630 | TGTCTTCAAACGCCTGGTGAG |
| TDF | 23,649,441 | 23,649,462 | CCGCCACGTTAGCATATTTCAC |
| TDP | 23,649,410 | 23,649,436 | TATCACATTCGGAGACTTTGGGTGGCA |
| TDR | 23,649,381 | 23,649,403 | AATCGCTTCCATCTCCTGTTCTC |
PCR Primers
| Primer | Sequence |
| SU | GGAAACATTCAAGCCAGAGC |
| SD | GAAAGATAACTGATGGCTTCTTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11486A-D1 | VGB6 | C57BL/6NTac | |
| 11486A-F9 | VGB6 | C57BL/6NTac | |
| 11486A-F2 | VGB6 | C57BL/6NTac | |
