| Gene | Wfdc13 |
| Chromosome | 2 |
| Deletion Start | 164,685,203 |
| Deletion End | 164,687,013 |
| Deletion Size | 1,811 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 164,685,596 | 164,685,616 | TCAGGCTAGGAGCTGTCTTAC |
| TUP | 164,685,618 | 164,685,640 | AGACCATGAGGCGCATCCCACTA |
| TUR | 164,685,654 | 164,685,673 | ACGAGGCTTACGACGTTGAC |
| TDF | 164,686,819 | 164,686,841 | CCACTTCATTCCAGAGTACATCC |
| TDP | 164,686,842 | 164,686,864 | TGGAACCTCCACCGTGCAGATCG |
| TDR | 164,686,885 | 164,686,904 | CCTCCTGCTGCGTACAGAAC |
PCR Primers
| Primer | Sequence |
| SU | TCCACATGAGAGGATGAAAG |
| SD | TATGAGTACGTTCAGAGAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 326 bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | 312 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11489A-G10 | VGB6 | C57BL/6NTac | |
| 11489A-G5 | VGB6 | C57BL/6NTac | |
| 11489A-B10 | VGB6 | C57BL/6NTac | |
