| Gene | Hbegf |
| Chromosome | 18 |
| Deletion Start | 36,514,060 |
| Deletion End | 36,505,198 |
| Deletion Size | 8,863 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,514,603 | 36,514,623 | GAGGGCAGTGAGGAACTCTTC |
| TUP | 36,514,576 | 36,514,600 | AGCTTTGGTAGATGTGGCCAGCACC |
| TUR | 36,514,532 | 36,514,553 | AGCTTCACAGGACCCAACATTC |
| TDF | 36,506,736 | 36,506,758 | GTGTCATTTCTTCACAGCCCTTC |
| TDP | 36,506,705 | 36,506,731 | CCTCAGGTACCACAGGAGAGGAGGTTA |
| TDR | 36,506,667 | 36,506,686 | CCACGCCCAACTTCACTTTC |
PCR Primers
| Primer | Sequence |
| SU | CTCTGAACAGACAGACGAAC |
| SD | ATCTTAACGACATGCAAGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11491A-A10 | VGB6 | C57BL/6NTac | |
| 11491A-D10 | VGB6 | C57BL/6NTac | |
| 11491A-E6 | VGB6 | C57BL/6NTac | |
| 11491A-F2 | VGB6 | C57BL/6NTac | |
