| Gene | Prelp |
| Chromosome | 1 |
| Deletion Start | 133,915,402 |
| Deletion End | 133,912,592 |
| Deletion Size | 2,811 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 133,915,372 | 133,915,389 | TCTGGCTCCTCCCACTTC |
| TUP | 133,915,349 | 133,915,371 | TCCTCATCTTGGCCTCAGTGGCC |
| TUR | 133,915,293 | 133,915,311 | GGCCTCGGCTTGGGTTTAC |
| TDF | 133,913,158 | 133,913,179 | TCCGACTCTGGGAAGAATGAAG |
| TDP | 133,913,125 | 133,913,153 | AAGATTGGGAGTTCAAGGTCAGCCTAGGA |
| TDR | 133,913,083 | 133,913,104 | TCTCTGAGCCTTTCTGGCAATG |
PCR Primers
| Primer | Sequence |
| SU | TGGATGTCACTCCTGGCAG |
| SD | AAGAGCAGAGGTCTTGGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 356 bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | 305 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11492A-A5 | VGB6 | C57BL/6NTac | |
| 11492A-C2 | VGB6 | C57BL/6NTac | |
| 11492A-C6 | VGB6 | C57BL/6NTac | |
| 11492A-C9 | VGB6 | C57BL/6NTac | |
| 11492A-D3 | VGB6 | C57BL/6NTac | |
| 11492A-D7 | VGB6 | C57BL/6NTac | |
