| Gene | Msx3 |
| Chromosome | 7 |
| Deletion Start | 140,048,996 |
| Deletion End | 140,047,589 |
| Deletion Size | 1,408 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 140,048,649 | 140,048,669 | ACTAGCCATTAACCGGCTCAG |
| TUP | 140,048,622 | 140,048,648 | TCCAAGAGCAATGATAACTGGCGGTGG |
| TUR | 140,048,561 | 140,048,579 | GCGGACCTCGACCAAGAAG |
| TDF | 140,048,193 | 140,048,215 | TGGAAGAAGGTCGGTCGAAATAG |
| TDP | 140,048,167 | 140,048,189 | TGTGCAGCCAGAGTTTGAGCGCT |
| TDR | 140,048,135 | 140,048,157 | GGGCACCTGAAAGGAAAGAGAAG |
PCR Primers
| Primer | Sequence |
| SU | TGGGCACGACAATGCTACC |
| SD | AATCGAGCCTCTCTGAAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 401 bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | 319 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11501A-G9 | VGB6 | C57BL/6NTac | |
| 11501A-G3 | VGB6 | C57BL/6NTac | |
| 11501A-E3 | VGB6 | C57BL/6NTac | |
