| Gene | Hspb3 |
| Chromosome | 13 |
| Deletion Start | 113,663,489 |
| Deletion End | 113,662,895 |
| Deletion Size | 595 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 113,663,295 | 113,663,315 | GACTCAGCCTCCACGGAGAAG |
| TUP | 113,663,271 | 113,663,293 | CACCCGGAGAAGGCAAATCCCGT |
| TUR | 113,663,236 | 113,663,255 | GGCAGGAACTGGACCACATC |
| TDF | 113,663,132 | 113,663,151 | ATCGCGGAGTTTCACCAGAC |
| TDP | 113,663,102 | 113,663,128 | ACAAACTGCCAGATGGAGTCGAAACCA |
| TDR | 113,663,078 | 113,663,099 | GACAGAGGATGGCAGACAAATC |
PCR Primers
| Primer | Sequence |
| SU | CTAAATGCAACTCGCAGAGG |
| SD | GTCCAAATGGCCTCTGTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 335 bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | 287 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11502A-A9 | VGB6 | C57BL/6NTac | |
| 11502A-B6 | VGB6 | C57BL/6NTac | |
| 11502A-B12 | VGB6 | C57BL/6NTac | |
| 11502A-D8 | VGB6 | C57BL/6NTac | |
| 11502A-G3 | VGB6 | C57BL/6NTac | |
| 11502A-H12 | VGB6 | C57BL/6NTac | |
