| Gene | Rnf113a1 |
| Chromosome | X |
| Deletion Start | 37,191,375 |
| Deletion End | 37,192,491 |
| Deletion Size | 1,117 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 37,191,568 | 37,191,585 | GCAAGCGACCCACAATCC |
| TUP | 37,191,588 | 37,191,610 | TGATCCAGAAGACGCGTGGCAGC |
| TUR | 37,191,617 | 37,191,635 | CGCCATAGGTTGCCTTCTG |
| TDF | 37,192,261 | 37,192,279 | CACCTCACGCTGCTATGTC |
| TDP | 37,192,280 | 37,192,302 | TGTGACCAGCAGACCAATGGCGT |
| TDR | 37,192,338 | 37,192,358 | CTGCTTCAGCTCGATGCTTTC |
PCR Primers
| Primer | Sequence |
| SU | CTTTACCTCTCTTCCGGCAG |
| SD | CACTTGTACCAACAGATAAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 361 bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | 333 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11503A-B2 | VGB6 | C57BL/6NTac | |
| 11503A-B3 | VGB6 | C57BL/6NTac | |
| 11503A-F1 | VGB6 | C57BL/6NTac | |
| 11503A-G5 | VGB6 | C57BL/6NTac | |
