| Gene | Klf14 |
| Chromosome | 6 |
| Deletion Start | 30,958,694 |
| Deletion End | 30,957,660 |
| Deletion Size | 1,035 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,958,415 | 30,958,437 | CGCTTTGGCTGATTTAAGCTGTG |
| TUP | 30,958,386 | 30,958,411 | CCCGGGAAGACTTCAGGGAAGACTCC |
| TUR | 30,958,348 | 30,958,368 | GGCTCGAAGCAGCTAGTTGAG |
| TDF | 30,957,996 | 30,958,013 | TGGCTCGACTGCGACAAG |
| TDP | 30,957,964 | 30,957,986 | CGTTCTGATGAGCTAGCCCGCCA |
| TDR | 30,957,918 | 30,957,936 | ACACAGCGGGCAGGAGAAG |
PCR Primers
| Primer | Sequence |
| SU | TCTGCCTGACACCCAGCTG |
| SD | GAGTTCATCCCTTCTCAAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 555 bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | 323 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11504A-H8 | VGB6 | C57BL/6NTac | |
| 11504A-E11 | VGB6 | C57BL/6NTac | |
| 11504A-B1 | VGB6 | C57BL/6NTac | |
| 11504A-H6 | VGB6 | C57BL/6NTac | |
