| Gene | Sprr1b |
| Chromosome | 3 |
| Deletion Start | 92,437,564 |
| Deletion End | 92,437,068 |
| Deletion Size | 497 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 92,437,523 | 92,437,540 | TGCACAGCTCCTCCACAG |
| TUP | 92,437,497 | 92,437,521 | TGCATGAGCAACAAGTGAAACAGCC |
| TUR | 92,437,438 | 92,437,457 | TGGTGTCACAGGGTGTCTTG |
| TDF | 92,437,171 | 92,437,188 | AGCCCTGCCAACCCATTG |
| TDP | 92,437,146 | 92,437,169 | TCCTGAGCCCTGTCCCTCAACTGT |
| TDR | 92,437,123 | 92,437,145 | TCTGTTGGGCTAGTATTGGAGTG |
PCR Primers
| Primer | Sequence |
| SU | CAATGTGAACCAATGCCATC |
| SD | GATGTCAACTGAAGGAGCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 66 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11507A-B3 | VGB6 | C57BL/6NTac | |
| 11507A-C6 | VGB6 | C57BL/6NTac | |
| 11507A-H9 | VGB6 | C57BL/6NTac | |
| 11507A-A5 | VGB6 | C57BL/6NTac | |
| 11507A-B1 | VGB6 | C57BL/6NTac | |
| 11507A-E10 | VGB6 | C57BL/6NTac | |
