| Gene | Eif3d |
| Chromosome | 15 |
| Deletion Start | 77,968,576 |
| Deletion End | 77,959,091 |
| Deletion Size | 9,486 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 77,968,236 | 77,968,255 | TCGCCTGACCTGTTGTCTTC |
| TUP | 77,968,210 | 77,968,234 | TTGTCCCTCACAGGTTGCAGACTGG |
| TUR | 77,968,179 | 77,968,201 | TGTACCTCTTGTCCTGGTATGTG |
| TDF | 77,959,761 | 77,959,781 | ACCACGTAAAGGACTCCTCAC |
| TDP | 77,959,737 | 77,959,759 | CCATGTCATCCTGGGCACCCAGC |
| TDR | 77,959,682 | 77,959,701 | CAGGCATTCTCCACGCTCAG |
PCR Primers
| Primer | Sequence |
| SU | CTAGAATCAGTGAGACATAGG |
| SD | GGCTTGTCATGGGGTTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 330 bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | 308 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11508A-A12 | VGB6 | C57BL/6NTac | |
| 11508A-E2 | VGB6 | C57BL/6NTac | |
