| Gene | Eif3f |
| Chromosome | 7 |
| Deletion Start | 108,934,416 |
| Deletion End | 108,941,545 |
| Deletion Size | 7,130 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 108,934,918 | 108,934,937 | TTGCCTTCCCGAGTGATCTG |
| TUP | 108,934,941 | 108,934,963 | TCAGCCGAATTGCCGTTAAGCCA |
| TUR | 108,935,038 | 108,935,059 | CCCTCTCAGTACCACGTTGTTG |
| TDF | 108,941,431 | 108,941,452 | GCCTCTCACTGCTTATCCTTTG |
| TDP | 108,941,455 | 108,941,479 | TTCCAGGACCTGCTGATGGTGACCT |
| TDR | 108,941,503 | 108,941,523 | TTTCTCGTTGAGGGCAATCTG |
PCR Primers
| Primer | Sequence |
| SU | GTGTAGATTGCTAGTTGCTTC |
| SD | CTGGCTTCCGTGCGAGTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 379 bp | - | + | + |
| TUF | TUR | 142 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | 298 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11510A-F5 | VGB6 | C57BL/6NTac | |
| 11510B-A4 | VGB6 | C57BL/6NTac | |
| 11510B-B5 | VGB6 | C57BL/6NTac | |
| 11510B-B7 | VGB6 | C57BL/6NTac | |
| 11510B-C10 | VGB6 | C57BL/6NTac | |
| 11510B-E5 | VGB6 | C57BL/6NTac | |
| 11510B-H5 | VGB6 | C57BL/6NTac | |
