11515   1110034A24Rik     Status: ES cell colonies screened / QC positives

Gene1110034A24Rik
Chromosome12
Deletion Start69,198,282
Deletion End69,189,824
Deletion Size8,459
Genome Build38
MGI IDMGI:1923566
NCBI Gene ID109065
BAC ID187I13
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 12

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF69,198,04469,198,062GCCATGTGCTGCGTACCAG
TUP69,198,01069,198,032AGCACCGCTGCTACGTGAACGTG
TUR69,197,98869,198,007CGCCTACCAGTGAGTTGCTG
TDF69,190,21169,190,228CTGTGGACCTGCAACACG
TDP69,190,18369,190,208TGTCCTGAGGTTCCTCACGTACTGTC
TDR69,190,13769,190,161TATAAACTCAGGATCCATTTTTGCC

PCR Primers

PrimerSequence
SUCAGTGGAGCCTATTTCTAGC
SDCTGACCTAAACTGACTGACC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev330 bp-++
TUFTUR75 bp++-
TDFTDR92 bp++-
NeoFwdSD215 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
11515B-A8VGB6C57BL/6NTac
11515B-B5VGB6C57BL/6NTac
11515B-F4VGB6C57BL/6NTac
11515B-G8VGB6C57BL/6NTac
11515B-G10VGB6C57BL/6NTac
11515B-H12VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
Larger Image

VelociGene Genotyping Strategy

Larger Image