Information regarding availability, timelines and delivery of materials can be found at www.komp.org or www.knockoutmouse.org.

11517 Senp5 Status: ES cell colonies screened / QC positives

Gene	Senp5
Chromosome	16
Deletion Start	31,990,431
Deletion End	31,963,388
Deletion Size	27,044
Genome Build	38

MGI ID	MGI:2443596
NCBI Gene ID	320213
BAC ID	48H9
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	Yes

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome 16

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	31,989,812	31,989,831	CACCACAGGAACAGGAGACC
TUP	31,989,782	31,989,809	TGATTCCAAAGCAGTTCCAGCTTAACCA
TUR	31,989,745	31,989,767	CTCATACATCATCAGAGACGCTC
TDF	31,963,787	31,963,808	GCACACCTCTAATCCCAGGAAG
TDP	31,963,757	31,963,783	CAGCAGATCTCTGTGAATTCGAGGCCA
TDR	31,963,719	31,963,741	GGCCCTAGCTGTACTGGATCTTG

PCR Primers

Primer	Sequence
SU	CACCAGAACCAGGTATAGAG
SD	TGTCTATATGCATGCGTTTGG
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	369 bp	-	+	+
TUF	TUR	87 bp	+	+	-
TDF	TDR	90 bp	+	+	-
NeoFwd	SD	329 bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+

Available Clones

ES Cell ordering information can be found at the KOMP Repository.

Clone Name	Parental ES Cell	Mouse Strain	Cassette Copy Number
11517A-D11	VGB6	C57BL/6NTac
11517A-E9	VGB6	C57BL/6NTac
11517A-F2	VGB6	C57BL/6NTac