| Gene | Dpm3 |
| Chromosome | 3 |
| Deletion Start | 89,266,737 |
| Deletion End | 89,267,025 |
| Deletion Size | 289 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 89,266,799 | 89,266,817 | CATGGGAGCACTGGGCTTG |
| TUP | 89,266,819 | 89,266,840 | AGTTGCCTTTCCCCTGCCGAGA |
| TUR | 89,266,862 | 89,266,881 | CAGCGGAGACCAACAGGTAG |
| TDF | 89,266,898 | 89,266,917 | CACGGTGGGCTATCGCGTAG |
| TDP | 89,266,920 | 89,266,942 | ACATTTCACGACTGCGAGGACGC |
| TDR | 89,266,956 | 89,266,975 | GCCTCCACGATCTGGCTCTG |
PCR Primers
| Primer | Sequence |
| SU | ACTTCCGGATGCTGATGCG |
| SD | GAAGGGAACAGAACACCATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 306 bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | 304 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11523A-G11 | VGB6 | C57BL/6NTac | |
| 11523A-C12 | VGB6 | C57BL/6NTac | |
| 11523A-E10 | VGB6 | C57BL/6NTac | |
| 11523A-D5 | VGB6 | C57BL/6NTac | |
| 11523A-F5 | VGB6 | C57BL/6NTac | |
| 11523A-C4 | VGB6 | C57BL/6NTac | |
