| Gene | Mrpl40 |
| Chromosome | 16 |
| Deletion Start | 18,876,520 |
| Deletion End | 18,872,281 |
| Deletion Size | 4,240 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 18,876,384 | 18,876,406 | CGCTAGTGCTCGCAGATTATTTC |
| TUP | 18,876,362 | 18,876,383 | TGGGCATGAGCCTACCGCATCT |
| TUR | 18,876,283 | 18,876,302 | GCACGACTTAGGCTTCACAC |
| TDF | 18,872,710 | 18,872,731 | CAAGGGTCCCTCTGCAATAGTG |
| TDP | 18,872,680 | 18,872,705 | TACTCTTTGGCCTCCCATGTTTGCTC |
| TDR | 18,872,649 | 18,872,667 | CCTGAGGTCGCTGTCTGTG |
PCR Primers
| Primer | Sequence |
| SU | TGTAAACACATAGGCCAGGC |
| SD | CCAGCATCTGAAAACCTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 387 bp | - | + | + |
| TUF | TUR | 124 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | 427 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11524B-C6 | VGB6 | C57BL/6NTac | |
| 11524B-C8 | VGB6 | C57BL/6NTac | |
| 11524B-D4 | VGB6 | C57BL/6NTac | |
