| Gene | Tatdn2 |
| Chromosome | 6 |
| Deletion Start | 113,697,699 |
| Deletion End | 113,710,188 |
| Deletion Size | 12,490 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 113,698,545 | 113,698,565 | TGAAGGAGGACACAGGTTCTG |
| TUP | 113,698,566 | 113,698,589 | AGAGCCCAGGTCAGGTGGTTCACA |
| TUR | 113,698,611 | 113,698,629 | CATGGGAGCCCGAGGAATG |
| TDF | 113,709,898 | 113,709,916 | AGGACTCTCCCTGCACCTG |
| TDP | 113,709,929 | 113,709,951 | TCTCTACAGGGCAGCCCCAAAGA |
| TDR | 113,710,029 | 113,710,047 | TCGGCACCTGGAAGACATC |
PCR Primers
| Primer | Sequence |
| SU | GCTTCTCGGAGCGCTGTG |
| SD | AGGTGAGTCCTTTAACACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 299 bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 150 bp | + | + | - |
| NeoFwd | SD | 347 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11526A-B1 | VGB6 | C57BL/6NTac | |
| 11526A-B10 | VGB6 | C57BL/6NTac | |
| 11526A-C4 | VGB6 | C57BL/6NTac | |
| 11526A-G10 | VGB6 | C57BL/6NTac | |
