| Gene | Smad5 |
| Chromosome | 13 |
| Deletion Start | 56,723,597 |
| Deletion End | 56,737,536 |
| Deletion Size | 13,940 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 56,724,152 | 56,724,175 | TGCCTGCTATTGATAAGCACTTTC |
| TUP | 56,724,182 | 56,724,207 | AACACCCATTTCCTTTCTGCGGCCTA |
| TUR | 56,724,233 | 56,724,255 | AGGACCTCGTGTGAATATGGAAC |
| TDF | 56,737,014 | 56,737,036 | ATGAGGGCGTGGGCTTTATTTAC |
| TDP | 56,737,037 | 56,737,065 | TCAGTGAGAATATGTATCACAGCACGGCA |
| TDR | 56,737,073 | 56,737,094 | CCAGACTATGATCCTTCACAGC |
PCR Primers
| Primer | Sequence |
| SU | TTAGACTGAATCAGGCTCCC |
| SD | AAGTACGTTCCAGTTTAAGATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 335 bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11528A-H3 | VGB6 | C57BL/6NTac | |
| 11528A-H4 | VGB6 | C57BL/6NTac | |
