11528   Smad5     Status: ES cell colonies screened / QC positives

GeneSmad5
Chromosome13
Deletion Start56,723,597
Deletion End56,737,536
Deletion Size13,940
Genome Build38
MGI IDMGI:1328787
NCBI Gene ID17129
BAC ID206O6
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 13

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF56,724,15256,724,175TGCCTGCTATTGATAAGCACTTTC
TUP56,724,18256,724,207AACACCCATTTCCTTTCTGCGGCCTA
TUR56,724,23356,724,255AGGACCTCGTGTGAATATGGAAC
TDF56,737,01456,737,036ATGAGGGCGTGGGCTTTATTTAC
TDP56,737,03756,737,065TCAGTGAGAATATGTATCACAGCACGGCA
TDR56,737,07356,737,094CCAGACTATGATCCTTCACAGC

PCR Primers

PrimerSequence
SUTTAGACTGAATCAGGCTCCC
SDAAGTACGTTCCAGTTTAAGATC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev335 bp-++
TUFTUR104 bp++-
TDFTDR81 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
11528A-H3VGB6C57BL/6NTac
11528A-H4VGB6C57BL/6NTac