| Gene | Tnfrsf13b |
| Chromosome | 11 |
| Deletion Start | 61,140,820 |
| Deletion End | 61,147,668 |
| Deletion Size | 6,849 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 61,141,551 | 61,141,571 | GCAGCGTGCTGGAATAACAAG |
| TUP | 61,141,576 | 61,141,604 | TTTAGTTCAAGAGGCTCAGACTCTAGGCA |
| TUR | 61,141,611 | 61,141,629 | GTGACCTCCCTGGCTTCTG |
| TDF | 61,146,985 | 61,147,005 | GTGGGTCACAAGCAAACTCTC |
| TDP | 61,147,006 | 61,147,028 | CCCACGCCCACCGTAAGTATGGA |
| TDR | 61,147,041 | 61,147,061 | TGCTGGTGATTGTCAGGGAAG |
PCR Primers
| Primer | Sequence |
| SU | GGTACACATGGAGGACAATG |
| SD | GTCATTCTCCATCTTTCTCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 364 bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | 293 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11530A-B6 | VGB6 | C57BL/6NTac | |
| 11530A-E8 | VGB6 | C57BL/6NTac | |
| 11530A-F10 | VGB6 | C57BL/6NTac | |
| 11530A-H6 | VGB6 | C57BL/6NTac | |
| 11530A-H7 | VGB6 | C57BL/6NTac | |
