| Gene | Pqbp1 |
| Chromosome | X |
| Deletion Start | 7,898,614 |
| Deletion End | 7,894,695 |
| Deletion Size | 3,920 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 7,898,124 | 7,898,147 | CACGGTGGCAGGTACAAATATCTC |
| TUP | 7,898,092 | 7,898,116 | AAGGAGGCTGAGCTACAAACATGGC |
| TUR | 7,898,059 | 7,898,078 | TTGCCATGTCTGGCCTTGAC |
| TDF | 7,894,823 | 7,894,843 | CAAGAGGAACGAGGCCAAGAC |
| TDP | 7,894,801 | 7,894,822 | AGGTGCTGACACCACGGCAGCT |
| TDR | 7,894,723 | 7,894,742 | TTTGGTTCGGGAGGCTTCTG |
PCR Primers
| Primer | Sequence |
| SU | TTCTGCTGATCCTAATTCTGG |
| SD | CAACAAGGGCTTGTTGTTTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 384 bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 121 bp | + | + | - |
| NeoFwd | SD | 247 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11531A-A8 | VGB6 | C57BL/6NTac | |
| 11531B-F2 | VGB6 | C57BL/6NTac | |
