| Gene | Sec61b |
| Chromosome | 4 |
| Deletion Start | 47,474,735 |
| Deletion End | 47,483,066 |
| Deletion Size | 8,332 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 47,474,875 | 47,474,894 | CCCGCGATGTTCACTGTTTG |
| TUP | 47,474,900 | 47,474,919 | TTCCCTCTGCAGCCGGGTCC |
| TUR | 47,474,956 | 47,474,974 | CACCGCTTTGCTGGGAGAG |
| TDF | 47,482,070 | 47,482,089 | CTGGACCACTGCTACCTCAC |
| TDP | 47,482,090 | 47,482,115 | CTGGCCCTGCCTGGTAATGCTTTGAT |
| TDR | 47,482,151 | 47,482,169 | TGCCCTCCCAGATACAACC |
PCR Primers
| Primer | Sequence |
| SU | TCGGCGTAATCTTTACAGGC |
| SD | ACTGCTACATCTTAAGGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 294 bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | 309 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11534A-B12 | VGB6 | C57BL/6NTac | |
| 11534A-C7 | VGB6 | C57BL/6NTac | |
| 11534A-G1 | VGB6 | C57BL/6NTac | |
