| Gene | Hint2 |
| Chromosome | 4 |
| Deletion Start | 43,656,436 |
| Deletion End | 43,654,295 |
| Deletion Size | 2,142 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,655,751 | 43,655,768 | CCCAGCAATGTGGGAGAC |
| TUP | 43,655,724 | 43,655,748 | TGGCAGCAGAATTACCAGGACTGCG |
| TUR | 43,655,682 | 43,655,702 | TGTGCAGCTTTCCTGTTAGTG |
| TDF | 43,654,793 | 43,654,811 | TTCCGTGACGTAGCTCCTC |
| TDP | 43,654,765 | 43,654,792 | AGGCTCCTGTGCACTTTCTGGTCATTCC |
| TDR | 43,654,715 | 43,654,733 | CCTGCTGGTCGTCCTCTTC |
PCR Primers
| Primer | Sequence |
| SU | GAACCTGAGTCCATTAGGAG |
| SD | TAGAGTGGACCATTAGACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 349 bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | 299 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11536A-A8 | VGB6 | C57BL/6NTac | |
| 11536A-B5 | VGB6 | C57BL/6NTac | |
| 11536A-F11 | VGB6 | C57BL/6NTac | |
