| Gene | Selm |
| Chromosome | 11 |
| Deletion Start | 3,514,930 |
| Deletion End | 3,517,164 |
| Deletion Size | 2,235 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 3,515,861 | 3,515,881 | CAGATGGCAGGTGCCTCTAAC |
| TUP | 3,515,886 | 3,515,912 | CCCTTAGGGTAGTTCTGAGCTGTTCCC |
| TUR | 3,515,931 | 3,515,948 | TGGCCTTTGGCCCTTGAG |
| TDF | 3,516,954 | 3,516,973 | GCTCCAGGACAGTGGCTAAG |
| TDP | 3,516,974 | 3,516,995 | TGCTGATCCCGCCCTTTCTCCA |
| TDR | 3,517,003 | 3,517,025 | GGTCATTTGGCTGAGTGGGATTC |
PCR Primers
| Primer | Sequence |
| SU | TATGGAGATTGTGAGAACTGG |
| SD | CACACCATCTACTCGCTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 363 bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | 309 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11537A-B11 | VGB6 | C57BL/6NTac | |
| 11537A-E9 | VGB6 | C57BL/6NTac | |
| 11537A-F5 | VGB6 | C57BL/6NTac | |
| 11537A-F9 | VGB6 | C57BL/6NTac | |
| 11537A-H3 | VGB6 | C57BL/6NTac | |
| 11537A-A8 | VGB6 | C57BL/6NTac | |
