| Gene | 4930519G04Rik |
| Chromosome | 5 |
| Deletion Start | 114,861,627 |
| Deletion End | 114,882,890 |
| Deletion Size | 21,264 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 114,861,882 | 114,861,902 | GCCCTTCTGGTCTTGGTTCAG |
| TUP | 114,861,905 | 114,861,924 | TGTGCCAGCACGGCTTTCGG |
| TUR | 114,861,935 | 114,861,954 | CCCAAGGGAGGAGTGTCAAC |
| TDF | 114,882,501 | 114,882,521 | GCCTCATTCACACCTGCATTC |
| TDP | 114,882,524 | 114,882,548 | TCCCTGAGTGCCTGAAAGTCACAGG |
| TDR | 114,882,573 | 114,882,592 | AGCTGCCTGGATCAAGTTTC |
PCR Primers
| Primer | Sequence |
| SU | ATCCATCATCAGGCAGTGTC |
| SD | TAGAAGTGCTTACGTTACAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 377 bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | 355 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11539B-F7 | VGB6 | C57BL/6NTac | |
| 11539D-H1 | VGB6 | C57BL/6NTac | |
| 11539D-E6 | VGB6 | C57BL/6NTac | |
