| Gene | Mansc1 |
| Chromosome | 6 |
| Deletion Start | 134,621,949 |
| Deletion End | 134,609,967 |
| Deletion Size | 11,983 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 134,621,627 | 134,621,648 | CTCCGTGGATCAGAAAGACAAG |
| TUP | 134,621,598 | 134,621,621 | CAGTGGCTGGGAGACTGTCCACAA |
| TUR | 134,621,573 | 134,621,594 | ACTAGGGTGGGATGGTGGAATG |
| TDF | 134,610,120 | 134,610,140 | GCCAGCATGGCCTTTCATTTG |
| TDP | 134,610,093 | 134,610,117 | AAGTGGCTTCTCATTGGGACCCTCC |
| TDR | 134,610,036 | 134,610,057 | CAACCAGCATCCTACCCAAGAG |
PCR Primers
| Primer | Sequence |
| SU | TTCTTCATAGGTATTTTCAGCC |
| SD | GAAGTAGGGCTATTCTTCAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 579 bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 105 bp | + | + | - |
| NeoFwd | SD | 213 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11540A-A9 | VGB6 | C57BL/6NTac | |
| 11540A-F12 | VGB6 | C57BL/6NTac | |
| 11540A-F7 | VGB6 | C57BL/6NTac | |
| 11540A-F3 | VGB6 | C57BL/6NTac | |
| 11540A-D4 | VGB6 | C57BL/6NTac | |
