| Gene | Pld2 |
| Chromosome | 11 |
| Deletion Start | 70,540,849 |
| Deletion End | 70,557,605 |
| Deletion Size | 16,757 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 70,541,432 | 70,541,450 | TTGGCTCGGTGAGTAGAAC |
| TUP | 70,541,452 | 70,541,473 | CGGTTGCTTCCCGTGAAAGGCA |
| TUR | 70,541,503 | 70,541,524 | AAGAGTCAGACAGACAGGAGTG |
| TDF | 70,557,380 | 70,557,398 | GGCTCTCCGGGAGTATGTG |
| TDP | 70,557,401 | 70,557,425 | TGTGGAGTCCTTGGCTACAGTCAGC |
| TDR | 70,557,453 | 70,557,470 | TAGGTGGCCCTGGATGTG |
PCR Primers
| Primer | Sequence |
| SU | TGGACCTGAATTTGGAATGAG |
| SD | GGAGGAAGAGTGAGATGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 357 bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | 408 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11541A-A11 | VGB6 | C57BL/6NTac | |
| 11541B-D1 | VGB6 | C57BL/6NTac | |
