| Gene | Phpt1 |
| Chromosome | 2 |
| Deletion Start | 25,574,836 |
| Deletion End | 25,573,561 |
| Deletion Size | 1,276 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,574,774 | 25,574,796 | CGGATGGCGTCTTCAAGTATGTG |
| TUP | 25,574,745 | 25,574,772 | TGATTCGAGTCCACTTAGCAGAGCCTTC |
| TUR | 25,574,709 | 25,574,728 | CGCACGATTTCCTTGCACTC |
| TDF | 25,573,705 | 25,573,725 | CAAACTGGCGGTCATCCTTTC |
| TDP | 25,573,683 | 25,573,704 | TCTAGGGTTACGGTCGTGCCCA |
| TDR | 25,573,621 | 25,573,640 | CAGCCCAGGTGACCTCATAG |
PCR Primers
| Primer | Sequence |
| SU | CCTTTAACTGGGACTCATGAG |
| SD | CTGAAGCTGAGCAGGTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 342 bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 105 bp | + | + | - |
| NeoFwd | SD | 297 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11544A-B3 | VGB6 | C57BL/6NTac | |
