| Gene | Nufip2 |
| Chromosome | 11 |
| Deletion Start | 77,686,213 |
| Deletion End | 77,709,735 |
| Deletion Size | 23,523 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 77,686,379 | 77,686,396 | ATCACCAGCAGCCTCACC |
| TUP | 77,686,399 | 77,686,420 | TACCTGCAGCATGGAGCCGAGG |
| TUR | 77,686,440 | 77,686,459 | GCTCATGTTTCAGCGGCTTG |
| TDF | 77,709,483 | 77,709,506 | CTGAAACAGGAAAGGGTTAGTCTC |
| TDP | 77,709,513 | 77,709,540 | TTGAAGTCTTCTCTGGGTGGTAGAGCAC |
| TDR | 77,709,543 | 77,709,567 | GCACTCCAAATAAATTCCCAACAAC |
PCR Primers
| Primer | Sequence |
| SU | GCATAGGGTAAGTGGCGTTG |
| SD | AAGTCTAATTACTTTCAGTTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 370 bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | 281 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11545A-H8 | VGB6 | C57BL/6NTac | |
| 11545A-C12 | VGB6 | C57BL/6NTac | |
