| Gene | A2ld1 |
| Chromosome | 14 |
| Deletion Start | 122,891,798 |
| Deletion End | 122,891,461 |
| Deletion Size | 338 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 122,891,747 | 122,891,765 | GGCCAACCCAACCACAAAG |
| TUP | 122,891,711 | 122,891,737 | ACCATTCACATGGCTTAGCAGCCTTCC |
| TUR | 122,891,671 | 122,891,693 | ATCACCAGTGGGAAGGACTCAAC |
| TDF | 122,891,558 | 122,891,580 | GTTGCGTTTCCTGGATGACTTTG |
| TDP | 122,891,529 | 122,891,549 | CCCAGCATGTACCAGCGCACA |
| TDR | 122,891,502 | 122,891,522 | CCACTCTAGCACCTGCACCTG |
PCR Primers
| Primer | Sequence |
| SU | GGCCTAGTGCCTCTTTCAG |
| SD | TGCTCCTTAAGACTAAAGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 363 bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | 367 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11549A-E5 | VGB6 | C57BL/6NTac | |
| 11549A-H2 | VGB6 | C57BL/6NTac | |
