11550 Arsa Status: ES cell colonies screened / QC no positives
| Gene | Arsa |
| Chromosome | 15 |
| Deletion Start | 89,475,719 |
| Deletion End | 89,472,893 |
| Deletion Size | 2,827 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 89,475,149 | 89,475,167 | TCAGGGCTTCCACCGATTC |
| TUP | 89,475,123 | 89,475,148 | CTGGGCATCCCATATTCCCATGACCA |
| TUR | 89,475,100 | 89,475,118 | AGAGGGTCCGGCTGGTTTG |
| TDF | 89,473,388 | 89,473,409 | AGCCCTCCAGGCTTTGAAACAC |
| TDP | 89,473,362 | 89,473,385 | CCAACTCCTCAAGGCCCAGTACGA |
| TDR | 89,473,317 | 89,473,334 | TCCTCGCCCTTGGCTATC |
PCR Primers
| Primer | Sequence |
| SU | GGCTCTGTGGAGTTACACC |
| SD | ATCACCTAACTCCAGACAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 358 bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | 317 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
