| Gene | B230312A22Rik |
| Chromosome | 4 |
| Deletion Start | 43,036,726 |
| Deletion End | 43,033,512 |
| Deletion Size | 3,215 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,036,315 | 43,036,335 | CCACCTCACCTTGGACTTCAG |
| TUP | 43,036,289 | 43,036,312 | ACCCAGAGTACACCCTGGCTCTCA |
| TUR | 43,036,249 | 43,036,269 | CCCAGTCTCTGGTGTGCAAAG |
| TDF | 43,033,771 | 43,033,790 | AGCTGGAGAAGCTCGGGTAG |
| TDP | 43,033,741 | 43,033,767 | TCTGTCCTCTGCCCTCAACAATCTCCC |
| TDR | 43,033,705 | 43,033,722 | GTGAGCACGTCAGCATCC |
PCR Primers
| Primer | Sequence |
| SU | GCAAGTCCATTGAGAAACTGG |
| SD | TCCCTAACTACCTAAGCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 400 bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | 320 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11551E-A2 | VGB6 | C57BL/6NTac | |
| 11551E-D3 | VGB6 | C57BL/6NTac | |
