| Gene | Taz |
| Chromosome | X |
| Deletion Start | 74,283,011 |
| Deletion End | 74,289,423 |
| Deletion Size | 6,413 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 74,283,245 | 74,283,267 | GAGCTTGGGACTGACTTCTCTTC |
| TUP | 74,283,269 | 74,283,295 | CAGAGTACATGAACCACCTCACCGTGC |
| TUR | 74,283,321 | 74,283,340 | CAGGGCCTCGGTTCTCAATG |
| TDF | 74,289,158 | 74,289,176 | GCTCCGGGCAGAGAACAAG |
| TDP | 74,289,177 | 74,289,200 | TCAGCTGTGAGTTTCCTCCCAGGG |
| TDR | 74,289,223 | 74,289,242 | TGGGAGTCTGAGGGACTGAG |
PCR Primers
| Primer | Sequence |
| SU | AGGACGCTTCCTCGAGTTG |
| SD | CTCCACCAAGTATAAGGGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 422 bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | 365 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11553C-F12 | VGB6 | C57BL/6NTac | |
| 11553B-F9 | VGB6 | C57BL/6NTac | |
| 11553C-F8 | VGB6 | C57BL/6NTac | |
