Information regarding availability, timelines and delivery of materials can be found at www.komp.org or www.knockoutmouse.org.

11554 Rcbtb1 Status: ES cell colonies screened / QC positives

Gene	Rcbtb1
Chromosome	14
Deletion Start	59,209,953
Deletion End	59,230,534
Deletion Size	20,582
Genome Build	38

MGI ID	MGI:1918580
NCBI Gene ID	71330
BAC ID	205P11
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	Yes

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome 14

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	59,210,023	59,210,046	GGATCTATCAGGAAAGCATGTGTC
TUP	59,210,049	59,210,071	TGGTACCTCTGCCAATGAGGCGA
TUR	59,210,089	59,210,109	TGCTTCCGGTCTTACCTCATC
TDF	59,230,333	59,230,352	GCCATGAAATGCCCGTGTTG
TDP	59,230,355	59,230,376	AGTCCCAGCGTCCCAGCACTGC
TDR	59,230,441	59,230,463	GACAAAGCCTCTTCAGTCTGTTC

PCR Primers

Primer	Sequence
SU	GCATACAGCTGTTTCTAGAATG
SD	GCTGAGTCCAGCACTGTAC
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	381 bp	-	+	+
TUF	TUR	87 bp	+	+	-
TDF	TDR	131 bp	+	+	-
NeoFwd	SD	315 bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+

Available Clones

ES Cell ordering information can be found at the KOMP Repository.

Clone Name	Parental ES Cell	Mouse Strain	Cassette Copy Number
11554B-B1	VGB6	C57BL/6NTac
11554B-B12	VGB6	C57BL/6NTac
11554B-D4	VGB6	C57BL/6NTac
11554B-H2	VGB6	C57BL/6NTac