| Gene | Ppp1r15a |
| Chromosome | 7 |
| Deletion Start | 45,525,379 |
| Deletion End | 45,523,714 |
| Deletion Size | 1,666 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 45,524,893 | 45,524,910 | TGGCTGAGCCCGCTTATC |
| TUP | 45,524,869 | 45,524,892 | CCACATCACAGCTGGAGGGTGGTC |
| TUR | 45,524,802 | 45,524,823 | GGAGCTATGGAAGCAGCAGAAG |
| TDF | 45,523,994 | 45,524,017 | GAGGAAGAGGACAGTGAGAATGTG |
| TDP | 45,523,964 | 45,523,990 | CCAGGTGACTCAGAAACAGCTGACTCA |
| TDR | 45,523,942 | 45,523,961 | GCTGAAGGCAGGGACTCTGG |
PCR Primers
| Primer | Sequence |
| SU | TGGGCTCTTGGTTCTAAGG |
| SD | CTCCAACACACTTCCTTAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 327 bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | 270 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11556A-D6 | VGB6 | C57BL/6NTac | |
| 11556A-E8 | VGB6 | C57BL/6NTac | |
| 11556A-F6 | VGB6 | C57BL/6NTac | |
| 11556A-G1 | VGB6 | C57BL/6NTac | |
