| Gene | Pqlc2 |
| Chromosome | 4 |
| Deletion Start | 139,309,575 |
| Deletion End | 139,306,710 |
| Deletion Size | 2,884 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 139,301,711 | 139,301,730 | GGTGAAGCAGGCTGAGATGG |
| TUP | 139,301,673 | 139,301,702 | ATACATCAAACCACAGGGAGGAGACTCTGT |
| TUR | 139,301,638 | 139,301,657 | CACCCTCACTTCTGGCTCTG |
| TDF | 139,300,383 | 139,300,403 | GCAGTCAACCCAAGGCATCTC |
| TDP | 139,300,355 | 139,300,377 | CGCTGTTCGCACTGGTGATGCTG |
| TDR | 139,300,335 | 139,300,353 | AGCCCGTACAGCGTGTTCC |
PCR Primers
| Primer | Sequence |
| SU | CCGTCAGAGGATAATACTGC |
| SD | AGCTTGTCTCCATGACTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 7013 bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | 7427 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11557A-A9 | VGB6 | C57BL/6NTac | |
| 11557A-B7 | VGB6 | C57BL/6NTac | |
| 11557A-C3 | VGB6 | C57BL/6NTac | |
| 11557A-D7 | VGB6 | C57BL/6NTac | |
| 11557A-G9 | VGB6 | C57BL/6NTac | |
| 11557A-H11 | VGB6 | C57BL/6NTac | |
