| Gene | Smpd1 |
| Chromosome | 7 |
| Deletion Start | 105,554,535 |
| Deletion End | 105,558,088 |
| Deletion Size | 3,554 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 105,554,772 | 105,554,791 | GGGTGGCAGAACCTCACTTG |
| TUP | 105,554,793 | 105,554,818 | CCCGCCTGCAAAGTCTTATTCACTGC |
| TUR | 105,554,832 | 105,554,852 | CCTTCACGCACTCACCTTCAG |
| TDF | 105,557,527 | 105,557,548 | CTGAGGCTGGTGTGACAGAAAG |
| TDP | 105,557,551 | 105,557,574 | ATGGAAGCCAAAGCAGGTCCTCCC |
| TDR | 105,557,583 | 105,557,606 | TGAGTGGTAAGGAAGGGAACAAAG |
PCR Primers
| Primer | Sequence |
| SU | GCTGAAGAAGGCGGATCAG |
| SD | CAGTACAGGGTTCAGAGGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 397 bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | 351 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11558A-A4 | VGB6 | C57BL/6NTac | |
| 11558A-C4 | VGB6 | C57BL/6NTac | |
| 11558A-E11 | VGB6 | C57BL/6NTac | |
