| Gene | Sycp3 |
| Chromosome | 10 |
| Deletion Start | 88,459,669 |
| Deletion End | 88,472,959 |
| Deletion Size | 13,291 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,460,090 | 88,460,109 | AGGGCCGAGGGAACTAGGAG |
| TUP | 88,460,110 | 88,460,134 | TCAAGGGCTAAAGCAGTCGGGAAGC |
| TUR | 88,460,188 | 88,460,207 | CCTCAGCCTGACCCTTCTTC |
| TDF | 88,472,039 | 88,472,062 | ACTAGGGCAGAGTTCAATCTATGG |
| TDP | 88,472,064 | 88,472,083 | ACCCATATGCTAGAAGAAGA |
| TDR | 88,472,084 | 88,472,105 | ACAACTCGTGGGTACTAGGTTC |
PCR Primers
| Primer | Sequence |
| SU | GAGGATCCATGAGCAAAGATG |
| SD | CTATACTTGGAACCTTGTACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 451 bp | - | + | + |
| TUF | TUR | 118 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | 434 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11560A-C8 | VGB6 | C57BL/6NTac | |
| 11560A-H1 | VGB6 | C57BL/6NTac | |
| 11560A-D10 | VGB6 | C57BL/6NTac | |
| 11560A-D8 | VGB6 | C57BL/6NTac | |
| 11560A-E5 | VGB6 | C57BL/6NTac | |
