11561   Cyp27a1     Status: ES cell colonies screened / QC positives

GeneCyp27a1
Chromosome1
Deletion Start74,713,634
Deletion End74,737,678
Deletion Size24,064
Genome Build38
MGI IDMGI:88594
NCBI Gene ID104086
BAC ID341H2
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 1

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF74,714,35074,714,373GAGCAGGCAAAGGAATTTCTGATG
TUP74,714,37574,714,398AATTGCACCTCGACACCCTGCCAG
TUR74,714,43274,714,450CCTGGCTGCTGGATTTCTC
TDF74,736,98074,737,000ACCTGGAAGCAGAGCGTAGAC
TDP74,737,00674,737,029TTTCCCTGCAGCCTCTACCCTGTG
TDR74,737,03574,737,056TGTGATGATCCGGGAGTTTGTG

PCR Primers

PrimerSequence
SUCATCTTGTCCACCTCTGCC
SDGTCAGATGCTGGATGACTGA
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev385 bp-++
TUFTUR101 bp++-
TDFTDR77 bp++-
NeoFwdSD344 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
11561A-B5VGB6C57BL/6NTac
11561A-C1VGB6C57BL/6NTac
11561A-H4VGB6C57BL/6NTac
11561A-F9VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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