| Gene | Nat1 |
| Chromosome | 8 |
| Deletion Start | 67,490,977 |
| Deletion End | 67,491,923 |
| Deletion Size | 947 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 67,491,333 | 67,491,352 | TGTGGATTCCGCCTATGGAG |
| TUP | 67,491,355 | 67,491,379 | TCCTACCAGATGTGGGAGCCTCTGG |
| TUR | 67,491,393 | 67,491,412 | GCACCTGAGGCTGATCCTTC |
| TDF | 67,491,607 | 67,491,629 | TCGCCAGCATCTGTGTTTGTAAG |
| TDP | 67,491,632 | 67,491,658 | CATCGTTCTGTTCCTTGCAGACCTCGG |
| TDR | 67,491,664 | 67,491,685 | TGGAGCCCACTAAACAGTGAAC |
PCR Primers
| Primer | Sequence |
| SU | TCGACATGGCCACTATGTTC |
| SD | TGGTTTCTTGTAAACCATGGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11571A-A3 | VGB6 | C57BL/6NTac | |
| 11571A-D5 | VGB6 | C57BL/6NTac | |
| 11571A-H3 | VGB6 | C57BL/6NTac | |
| 11571A-F10 | VGB6 | C57BL/6NTac | |
| 11571A-F2 | VGB6 | C57BL/6NTac | |
| 11571A-H9 | VGB6 | C57BL/6NTac | |
